Precipitate Exosomes from Biofluids
Discover microRNA and Protein Biomarkers in Patient Bio-fluids Exosomes are 40 –100 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs depending upon the tumor from which they are secreted. SBI's ExoQuick exosome precipitation reagent makes microRNA and protein biomarker discoveries simple, reliable and quantitative. Enrich for circulating exosomal microRNAs with ExoQuick and accurately profile them using SBI’s QuantiMir qPCR arrays.
- No time-consuming ultracentrifugation
- Less expensive than costly Antibodies and beads
- More effective than any other method
- Use as little as 100 µl of serum or biofluid
Please contact our customer service team for FREE TRIAL SAMPLES of the System Biosciences Exoquick Exosome Isolation Solution at customerservice@aust-biosearch.com.au or free call on 1800 858 797 (Subject to availability).
For a limited time, System Biosciences is offering the ExoQuick Exosome Isolation Solution at 50% off the list price. Offer is valid until the 1st of July, 2010. Please contact our office for pricing and availability.
MicroRNA Libraries Screen for microRNA phenotypes | Pooled lentivirus libraries • All microRNA clones represented
• Overexpress with Lenti-miRs
• Knockdown with miRZips
• Transduce all cell types, even stem cells
• Identify microRNA effectors using PCR |
Perform one transduction and easily identify the microRNAs involved in your phenotypic screen The Lenti-miR™ virus library contains a pool of SBI’s microRNA precursor clones and the miRZip™ virus library contains all of the miRZip anti-miR constructs in a ready-to-infect lentiviral preparation. The OncoMir virus library contains 141 of the most studied microRNAs involved in oncogenesis. Each virus within the Lenti-miR or OncoMir virus pool will express an individual microRNA precursor in its native context while preserving putative hairpin structures to ensure biologically relevant interactions with endogenous processing machinery and regulatory partners. Each virus in the miRZip library pool will express an individual anti-miR shRNA to permanently inhibit a specific microRNA. Details of the individual microRNA precursor clones and the list of the microRNAs which comprise the virus pool can be found on the Lenti-miR collection. The microRNA clones in the OncoMir virus library can be downloaded in the OncoMir virus Library List of Clones (PDF). More information on how miRZips work and the lists of the individual miRZip anti-miR clones in the virus pool can be found on the miRZip webpages.  | The microRNA or microRNAs responsible for generating the phenotypes of interest may be recovered through simple genomic PCR using the included lentivector-specific primers followed by direct sequencing. All microRNA precursor clones are about 500-700 bp in size and each miRZip shRNA segment amplified will be about 240 bp. |  | Quality Control of Lenti-miR Virus Library Infectious titer is determined through real-time qPCR using SBI’s UltraRapid Lentiviral Titer Kit. Shown below is GFP expression in HT1080 cells transduced with the Lenti-miR Virus Library.  Lenti-miR Precursor Library Kit Components - Aliquots of pooled Lenti-miR Virus Library (LVL)
- Tube of positive infection control virus (CD511VB-1)
- Tube of LVL PCR Primer Mix (10 μM of each primer)
- Each kit comes with user manual.
PMIRHPLVA-1 (4 pooled virus aliquots: 5 to 10 screens)
PMIRHPLVAHT-1 (10 pooled virus aliquots: 12 to 40 screens) miRZip anti-miR Library Kit Components - One Aliquot of pooled miRZip Virus Library (MZIPPLVA)
- Tube of positive infection control virus for Scramble hairpin (MZIP000-VA-1)
- Tube of GNH PCR Primer Mix (10 μM of each primer)
- Each kit comes with user manual.
MZIPPLVA-1 (1 pooled virus aliquot: 2 to 5 screens) |
* Prices listed on the System Biosciences website are in U.S. Currency. For Australian pricing, please call our office
