DiscoverX provides technologies which enable the researcher to interrogate various signalling pathways, including various GPCRs, kinases, proteases and nuclear hormone receptors. DiscoverX also provides custom assay development.

Nuclear Hormone Receptors (NHRs)

Nuclear Hormone Receptors (NHRs) are a superfamily of ligand-dependent transcription factors that control diverse aspects of growth, development and homeostasis, making them exciting and important targets for drug discovery.

DiscoveRx offers series of assays that measure NHR translocation and NHR-coactivator interaction. These unique assays are chemiluminescent and do not require lengthy 16-24 hour compound incubations characteristic of standard reporter gene assays.

Product Features

• Simple, no-wash, non-radioactive assays - Direct 6 hour assays for NHR 
 activation and signaling
•Target-specific cell-based assays or binding assay formats for purified NHRs 
•Understand effect of compound on multiple pathways with both translocation and 
protein:protein interaction approach 
•Full representation of steroid hormone receptors, reproductive hormones, etc.

NHR Assays Selection Guide

Clonal Cell Lines

PathHunter™ Nuclear Translocation Assays

PathHunter™ Nuclear Translocation assays are non-imaging whole cell assays to analyze translocation of NHR upon ligand stimulation.
Read more»

Targets: GR, AR, LXRα, VDR, MR, PRβ

PathHunter™ Protein:protein Interaction

PathHunter™ NHR_PRO assays are whole cell functional assays, based on the interaction between NHR and Steroid Receptor Coactivator Peptides (SRCP). The assay is ideal for novel inhibitor screening and pre-toxicity analysis. Read more» 

Targets: GR, MR, AR, THRα, THRβ, RARα, RARβ, RXRα, RXRγ, ERRα, LXRα, LXRβ, PPARα, PPARγ, PPARδ, FXR, ERα, PRα, PRβ, RORγ

Biochemical

HitHunter™ Nuclear 
Receptor Assays

Homogeneous immunoassays where analogs of the nuclear hormone compete with ED-nuclear hormone for binding to the nuclear hormone receptor. Read more»

Targets: PR, ER

Other

PathHunter™ 
Detection Reagents

Chemiluminescent detection reagents for use with all PathHunter™ 
Cell Lines. Kits available for either Glow or Flash luminescence. 
Read more»

PathHunter™ Translocation Toolbox Products

Create your own PathHunter protein trafficking assay using our validated parental cell lines and ProLabel expression vectors. Read more»

Don't find your assay?

Talk to our experts, we excel in Custom Assay Development possibilities!
Or you may be interested in NHR screening and profiling services.

 * Prices listed on the DiscoverX website are in U.S. Currency. For Australian pricing, please call our office 

Website: http://www.discoverx.com/

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