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Marligen Biosciences

From DNA and RNA purification kits to assays and testing services for SNP genotypingmicroRNA profiling and transcription factor activity analysis, Marligen supplies life science researchers worldwide with high-quality, easy-to-use tools for molecular and cell biology.

Marligen is a leader in the development of high performance multiplex assays for the Luminex xMAP® platform. Multiplexed assays for microRNA expressiontranscription factor binding activity, and SNP genotyping profile biological samples in an efficient, cost-effective manner. 

Vantage™ microRNA Multiplex Detection Panels

The Vantage™ microRNA Detection Panels offers researchers a fast and simple method for profiling the expression levels of up to 100 microRNAs in hundreds of samples in just a few hours. This cost effective method measures the expression of microRNAs known to be relevant in disease for less than $2/miRNA.Download full list of Vantage microRNA Detection Panels.

 

Advantages:

  • Configuration of assays on xMAP® multiplex bead array allows analysis of up to 100 microRNAs in one sample
  • 96-well format allows for hundreds of samples to be profiled in one day
  • Cost effective - < $2/microRNA/sample
  • Simple hybridization protocol can be completed in less than 2 hours
  • Detects microRNA profiles in all types of RNA samples including enriched or degraded samples*
  • All multiplexes include internal assay control for sample normalization
  • Flexible bead-array allows customer-specified panels to be quickly formatted (learn more)

Vantage™ microRNA Detection Panels can detect all RNA sample types. Samples must be labeled withVantage™ microRNA Labeling Kit prior to assaying on Detection Panels.

 

Vantage™ microRNA Multiplex Detection Panels

Item Name Catalog Number  
Oncology Detection Panel 1 (47-plex) AM100045 (11831-050)  
Pancreatic Cancer Panel 1 (12-plex) AM100046 (11832-050)  
Breast Cancer Panel 1 (8-plex) AM100047 (11833-050)  
Ovarian Cancer Panel (8-plex) AM100048 (11834-050)  
Cardiac Panel 1 (10-plex) AM100049 (11835-050)  
Diabetes Panel 1 (11-plex) AM100050 (11836-050)  
Hypoxia Panel 1 (10-plex) AM100051 (11837-050)  
Prostate Cancer Panel (18-plex) AM100052 (11838-050)  

1. The sequences and nomenclature of the mature microRNAs are extracted from The miRBase Sequence Database version 14, released in September 2009, in Sanger Institute in UK (miRBase).

2. All of the microRNAs detected by the Vantage™ Detection Panels are designated by human sequence nomenclature.Download full list of Vantage™ microRNA Detection Panels above to obtain homology with mouse and rat. For other species, please contact us.

3. All detection panels include an normalization control. Bead 49 detects 5.8S RNA that is ubiquitously expressed in mammalian cells. A signal of 5000-10000 MFI is typically observed when using 500 – 1000 ng of high quality (RIN >8) total RNA.

Simple hybridization protocol can be completed in less than 2 hours

Vantage™ microRNA Detection Protocol

 

Perfect for Comparing Many Normal and Disease Samples at the Same Time

 

Comparison of methods for detecting microRNA expression levels

  Vantage™ Detection Panels Microarrays qRT-PCR
RT Step no no yes
Amplification no no yes
Multiplex yes yes no
<3 hour protocol yes no yes
Sample throughput high low medium
Flexible assay format yes no yes

 

Don’t see the multiplex you need. Try our custom option:

Select any microRNA assay from our list of developed assays and we will make you a multiplex assay that meets your specific research needs. There is no additional charge for this service and the turnaround time for delivery of your custom multiplex assay is typically 5-7 business days. (Time frame applicable in the US only).

 * Prices listed on the Marligen website are in U.S. Currency. For Australian pricing, please call our office. Availability times listed on the Marligen website are only applicable in the U.S.

 

Website: http://www.marligen.com/index.html

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