Genotyping without DNA isolation
Viagen Biotech Inc. is a leading provider of innovative DNA extraction technologies and products for life science research. Viagen’s products are sold to academic research markets, biotechnology companies, and pharmaceutical companies. Through a global network of more than 30 international distributors, the company offers quality products and services to a worldwide network of research scientists in life science. The patent-pending components of the Viagen DirectPCR® DNA Extraction System allows DNA extracts to be compatible with genomic PCR for genotyping. This is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells.
Genotyping without DNA isolation. Viagen Biotech DirectPCR Lysis Reagents are a full line of single-tube systems for rapid, convenient, and reliable preparation of DNA from mouse tails, ears, yolk sacs, and culture cells. The innovative system developed by Viagen Biotech allows the resulting DNA crude extracts to be ready for genomic PCR for genotyping in less time and less hands-on involvement. The major steps include lysis using these DirectPCR Reagents, incubation for 45 minutes at 85°C, and PCR using 1 µl lysates.
Viagen Biotech offers high quality, genomic PCR grade Proteinase K products in both solution and powder forms at affordable costs. Their Proteinase K products are free of detectable DNase and RNase.
Crude extracts of biological samples are incompatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), largely due to inhibitors contained in crude extracts. E-Prep® DNA Extraction System is a single-tube system for rapid preparation of DNA from various biological samples and fluids such as blood, hair, buccal swab, and bloodstain; which allow that the resulting DNA extracts are compatible with genomic PCR for genotyping.
Nucleic Acid Staining Solution
RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid stain, an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. The Redsafe solution has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe™ when bound to DNA is centered at 537 nm.
Viagen offers the most affordable DNA markers compared to other leading companies. Pre-mixed with loading dye and ready-to-use, highly visible bands with convenient spacing—few at increased intensity for easy identification. Can be stained with RedSafe™ Nucleic Acid Staining Solution (Cat No. 21141), EtBr or other DNA stains.
* Prices listed on Advanced Viagen’s website are in U.S. Currency. For Australian pricing, please call our office or contact us at firstname.lastname@example.org
|Product Name||Product Number||Supplier||Size|
|DirectPCR Lysis Reagent (cell)||301-C||Viagen Biotech||VIEW DETAILS|
|DirectPCR Lysis Reagent (cell)||302-C||Viagen Biotech||VIEW DETAILS|
|DirectPCR Lysis Reagent (mouse ear)||401-E||Viagen Biotech||VIEW DETAILS|
|DirectPCR Lysis Reagent (mouse ear)||402-E||Viagen Biotech||VIEW DETAILS|
|DirectPCR Lysis Reagent (mouse tail)||101-T||Viagen Biotech||VIEW DETAILS|
|DirectPCR Lysis Reagent (mouse tail)||102-T||Viagen Biotech||VIEW DETAILS|
|DirectPCR Lysis Reagent (yolk sac)||201-Y||Viagen Biotech||VIEW DETAILS|
|DirectPCR Lysis Reagent (yolk sac)||202-Y||Viagen Biotech||VIEW DETAILS|
|Proteinkinase K Solution||501-PK||Viagen Biotech||VIEW DETAILS|